论文
| 论文编号: | |
| 第一作者所在部门: | |
| 中文论文题目: | Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the SH3 domain of human AHI1 |
| 英文论文题目: | Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the SH3 domain of human AHI1 |
| 作者: | zhuliang shi,Ning Liang,Wei Xu,Kuai Li,Cuoqing Sheng,Jingsong Liu,Aimin Xu,Xiao-Jiang Li and Donghai Wu |
| 论文出处: | |
| 刊物名称: | Acta Crystallographica Section F |
| 年: | 2009-03-03 |
| 卷: | F65 |
| 期: | |
| 页: | 361-363 |
| 联系作者: | Donghai Wu |
| 收录类别: | |
| 影响因子: | 0.365 |
| 摘要: | The SH3 domain of human AHI1 was cloned and expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and was crystallized using the sitting-drop vapour-diffusion method at 293 K. A complete data set was collected to 2.5 A ° resolution at 110 K. The crystal belonged to space group P41212, with unit-cell parameters a = 67.377, b = 67.377, c = 98.549 A ° . |
| 英文摘要: | |
| 外单位作者单位: | |
| 备注: | People’s Republic of China, bDepartment of Life Sciences, University of Science and Technology of China, People’s Republic of China, cDepartment of Medicine, The University of Hong Kong, Hong Kong, and dDepartment of Human Genetics, Emory University School of Medicine, Atlanta, Georgia, USA |