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中文论文题目: | Aptamer?Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells |
英文论文题目: | Aptamer?Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells |
作者: | Guodong Liu*, Xun Mao, Joseph A. Phillips, Hui Xu, Weihong Tan* and Lingwen Zeng |
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刊物名称: | Anal. Chem |
年: | 2009 |
卷: | 81 |
期: | 24 |
页: | 10013–10018 |
联系作者: | Lingwen Zeng |
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影响因子: | 5.712 |
摘要: | We report an aptamer−nanoparticle strip biosensor (ANSB) for the rapid, specific, sensitive, and low-cost detection of circulating cancer cells. Known for their high specificity and affinity, aptamers were first selected from live cells by the cell-SELEX (systematic evolution of ligands by exponential enrichment) process. When next combined with the unique optical properties of gold nanoparticles (Au-NPs), ANSBs were prepared on a lateral flow device. Ramos cells were used as a model target cell to demonstrate proof of principle. Under optimal conditions, the ANSB was capable of detecting a minimum of 4000 Ramos cells without instrumentation (visual judgment) and 800 Ramos cells with a portable strip reader within 15 min. Importantly, ANSB has successfully detected Ramos cells in human blood, thus providing a rapid, sensitive, and low-cost quantitative tool for the detection of circulating cancer cells. ANSB therefore shows great promise for in-field and point-of-care cancer diagnosis and therapy. |
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