专家人才
- 姓名： 赖良学
- 性别： 男
- 职称： 研究员
- 学历： 博士
- 电话：
- 传真：
- 电子邮件: lai_liangxue@gibh.ac.cn
- 通讯地址 广州萝岗区科学城开源大道190号
1984年在四川畜牧兽医学院获学士学位，1987年在中国人民解放军兽医大学获硕士学位，1995年在东北农业大学获博士学位。1995年至1997年在中国人民解放军农牧大学从事博士后研究。1998年赴美国密苏里大学动物研究中心从事博士后研究，2002年晋升为美国密苏里大学动物研究中心研究助理教授。2007年7月回国，担任中国科学院广州生物医药与健康研究院研究员、博士生导师。
简历：
1.大动物干细胞：大动物多能干细胞分离培养体系的建立与宠物干细胞治疗的有效性及安全性研究；
2.大动物克隆：在探索体细胞重编程机制的基础上，优化大动物体细胞克隆技术，提高体细胞重编程效率，开展猪、犬、猫以及家兔等大动物的克隆。
3.新型基因修饰技术开发与优化：建立精准、高效和安全的大动物基因编辑技术体系。
4. 基因修饰大动物的培育：将体细胞克隆技术与基因修饰技术相结合，培育在农业和生物医药有重要价值的基因修饰大动物模型。
5.人源化基因修饰大动物模型的转化应用：利用含有人源化功能蛋白分子基因修饰猪制备可用于人类疾病治疗的生物活性制品；利用免疫原性人源化的猪器官开展异种器官移植的临床前研究；利用人源化基因修饰大动物疾病模型开展基因治疗、干细胞治疗以及创新药物治疗的有效性与安全性评估。
研究领域：
1.国家自然科学基金区域创新发展联合基金重点支持项目（2024/01-2027/12），项目负责人；
2.国家重点研发计划“干细胞研究与器官修复”重点专项项目（2022/12-2027/11），项目负责人；
3.中国科学院“器官重建与制造”战略性先导科技专项（A类）（2017/12-2022/11），课题负责人；
4.国家重点研发计划“干细胞及转化研究”重点专项项目（2017/07-2022/06），项目负责人；
5.国家重大科学研究计划（973）项目（2011/01-2015/08），项目负责人；
6.国家高技术研究发展计划（863）重点项目（2006/12-2010/12），项目负责人。
承担科研项目情况：
- 广东省遗传学会理事长；广东省实验动物学会副理事长；中国遗传学会理事；中国实验动物学会理事；中国实验动物学会小型猪专业委员会副主任委员；中国合格评定国家认可委员会（CNAS）实验室专门委员会实验动物专业委员会委员；国家禽类实验动物资源库专家委员会委员；
社会任职：
国家海外杰出青年基金获得者；
中国科学院引才计划入选者
国务院政府特殊津贴；
广东省“南粤百杰”；
广东省科学技术奖一等奖（第一完成人）；
吉林省科学技术奖一等奖（第一完成人）；
广州市科学技术奖一等奖（第一完成人）；
获2002年度世界最新医学技术大奖；
两项成果入选美国《发现》杂志评出年度世界100大科技新闻。
获奖及荣誉：
1. Weikai Ge#, Shixue Gou#, Xiaozhu Zhao#, Qin Jin#, Zhenpeng Zhuang, Yu Zhao, Yanhui Liang, Zhen Ouyang, Xiaoyi Liu, Fangbing Chen, Hui Shi, Haizhao Yan, Han Wu*, Liangxue Lai*, Kepin Wang*, In vivo evaluation of guide-free Cas9-induced safety risks in a pig model, Signal Transduction and Targeted Therapy, 2024. Accepted.
2. Jiaowei Wang#, Wenguang Xie#, Nan Li#, Wenjuan Li#, Zhishuai Zhang#, Nana Fan, Zhen Ouyang, Yu Zhao, Chengdan Lai, Hao Li, Mengqi Chen, Longquan Quan, Yunpan Li, Yu Jiang, Wenqi Jia, Lixin Fu, Md Abdul Mazid, Yanling Zhu, Patrick H Maxwell, Guangjin Pan*, Miguel A Esteban*, Zhen Dai*, Liangxue Lai*, Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation, Cell Stem Cell, 2023, 30(9):1235-1245.
3. Sen Yan#*, Xiao Zheng, Yingqi Lin, Caijuan Li, Zhaoming Liu, Jiawei Li, Zhuchi Tu, Yu Zhao, Chunhui Huang, Yizhi Chen, Jun Li, Xichen Song, Bofeng Han, Wei Wang, Weien Liang, Liangxue Lai*, Xiao-Jiang Li*, Shihua Li*, Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington's disease, Nature Biomedical Engineering, 2023, 7(5):629-646.
4. Qin Jin#, Xiaoyi Liu#, Zhenpeng Zhuang#, Jiayuan Huang#, Shixue Gou, Hui Shi, Yu Zhao, Zhen Ouyang, Zhaoming Liu, Lei Li, Junjie Mao, Weikai Ge, Fangbing Chen, Manya Yu, Yezhi Guan, Yinghua Ye, Chengcheng Tang, Ren Huang, Kepin Wang*, Liangxue Lai*, Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation, Genome Biology, 2023, 24:8.
5. Qin Jin#, Xiaoyu Yang, Shixue Gou, Xiaoyi Liu, Zhenpeng Zhuang, Yanhui Liang, Hui Shi, Jiayuan Huang, Han Wu, Yu Zhao, Zhen Ouyang, Quanjun Zhang, Zhaoming Liu, Fangbing Chen, Weikai Ge, Jingke Xie, Nan Li, Chengdan Lai, Xiaozhu Zhao, Jiaowei Wang, Meng Lian, Lei Li, Longquan Quan, Yinghua Ye, Liangxue Lai*, Kepin Wang*, Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression, Science China Life Sciences, 2022, 65(11):2269-2286.
6. Yanhui Liang#, Jingke Xie#, Quanjun Zhang#, Xiaomin Wang#, Shixue Gou, Lihui Lin, Tao Chen, Weikai Ge, Zhenpeng Zhuang, Meng Lian, Fangbing Chen, Nan Li, Zhen Ouyang, Chengdan Lai, Xiaoyi Liu, Lei Li, Yinghua Ye, Han Wu, Kepin Wang*, Liangxue Lai*, AGBE: A dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns, Nucleic Acids Research, 2022, 50(9):5384-5399.
7. Xiaomin Wang#, Yanhui Liang#, Jianping Zhao#, Yuan Li, Shixue Gou, Min Zheng, Juanjuan Zhou, Quanjun Zhang, Jidong Mi*, Liangxue Lai*, Generation of permanent neonatal diabetes mellitus dogs with glucokinase point mutations through base editing, Cell Discovery, 2021, 7:92.
8. Jingke Xie#, Weikai Ge#, Nan Li#, Qishuai Liu#, Fangbing Chen, Xiaoyu Yang, Xingyun Huang, Zhen Ouyang, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Shixue Gou, Han Wu, Chengdan Lai, Nana Fan, Qin Jin, Hui Shi, Yanhui Liang, Ting Lan, Longquan Quan, Xiaoping Li, Kepin Wang*, Liangxue Lai*, Efficient base editing for multiple genes/loci in pigs using base editors, Nature Communications, 2019, 10:2852.
9. Sen Yan#, Zhuchi Tu#, Zhaoming Liu#, Nana Fan, Huiming Yang, Su Yang, Weili Yang, Yu Zhao, Zhen Ouyang, Chengdan Lai, Huaqiang Yang, Li Li, Qishuai Liu, Hui Shi,2 Guangqing Xu, Heng Zhao, Hongjiang Wei, Zhong Pei, Shihua Li*, Liangxue Lai*, Xiao-Jiang Li*, A huntingtin knock-in pig model recapitulates features of selective neurodegeneration in Huntington’s disease, Cell, 2018, 173(4):989-1002.
10. Kepin Wang#, Qin Jin#, Degong Ruan#, Yi Yang, Qishuai Liu, Han Wu, Zhiwei Zhou, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Jiangyun Peng, Chengdan Lai, Nana Fan, Yanhui Liang, Ting Lan, Nan Li, Xiaoshan Wang, Xinlu Wang, Yong Fan, Pieter A. Doevendans, Joost P.G. Sluijter, Pentao Liu, Xiaoping Li*, Liangxue Lai*, Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing, Genome Research, 2017, 27(12): 2061-2071.
11.Yi Yang#, Kepin Wang#, Han Wu, Qin Jin, Degong Ruan, Zhen Ouyang, Bentian Zhao, Zhaoming Liu, Yu Zhao, Quanjun Zhang, Nana Fan, Qishuai Liu, Shimei Guo, Lei Bu, Yong Fan, Xiaofang Sun, Xiaoping Li*, Liangxue Lai*, Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering, Journal of Molecular Cell Biology, 2016, 8(2): 174-177.
12. Guohao Wang#, Huaqiang Yang#, Sen Yan#, Chuan-En Wang, Xudong Liu, Bentian Zhao, Zhen Ouyang, Peng Yin, Zhaoming Liu, Yu Zhao, Tao Liu, Nana Fan, Lin Guo, Shihua Li, Xiao-Jiang Li*, Liangxue Lai*, Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain, Molecular Neurodegeneration, 2015, 10:42.
13. Huaqiang Yang#, Guohao Wang#, Haitao Sun#, Runzhe Shu#, Tao Liu, Chuan-En Wang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Zhen Ouyang, Dongshan Yang, Jiao Huang, Yueling Zhou, Shihua Li, Xiaodan Jiang*, Zhicheng Xiao*, Xiao-Jiang Li*, Liangxue Lai*, Species-dependent neuropathology in transgenic SOD1 pigs, Cell Research, 2014, 24(4):464-481.
14. Xiaoping Li#, Yi Yang#, Lei Bu, Xiaogang Guo, Chengcheng Tang, Jun Song, Nana Fan, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Xiaoling Yi, Longquan Quan, Songcai Liu, Zhenguo Yang, Hongsheng Ouyang, Y Eugene Chen, Zhong Wang*, Liangxue Lai*, Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing, Cell Research, 2014, 24(4):501-504.
15. Jun Song, Juan Zhong, Xiaogang Guo, Yongqiang Chen, Qingjian Zou, Jiao Huang, Xiaoping Li, Quanjun Zhang, Zhiwu Jiang, Chengcheng Tang, Huaqiang Yang, Tao Liu, Peng Li, Duanqing Pei, Liangxue Lai, Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs, Cell Research, 2013, 23(8):1059-1062.
16. Nana Fan#, Jijun Chen#, Zhouchun Shang#, Hongwei Dou, Guangzhen Ji, Qingjian Zou, Lu Wu, Lixiazi He, Fang Wang, Kai Liu, Na Liu, Jianyong Han, Qi Zhou, Dengke Pan, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Lin Lin, Chongming Zhong, Quanlei Wang, Shouqi Wang, Ying Xu, Jing Luan, Yu Liang, Zhenzhen Yang, Jing Li, Chunxia Lu, Gábor Vajta, Ziyi Li, Hongsheng Ouyang, Huayan Wang, Yong Wang, Yang Yang, Zhonghua Liu, Hong Wei, Zhidong Luan, Miguel A Esteban, Hongkui Deng, Huanming Yang, Duanqing Pei, Ning Li, Gang Pei, Lin Liu, Yutao Du*, Lei Xiao*, Liangxue Lai*, Piglets cloned from induced pluripotent stem cells, Cell Research, 2013, 23(1):162-166.
17. Dongshan Yang#, Huaqiang Yang#, Wei Li, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Nana Fan, Jun Song, Jiangtian Tian, Feng Li, Jifeng Zhang, Lin Chang, Duanqing Pei, Y Eugene Chen*, Liangxue Lai*, Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning, Cell Research, 2011, 21(6):979-982.
18. Liangxue Lai#, Jing X Kang#, Rongfeng Li, Jingdong Wang, William T Witt, Hwan Yul Yong, Yanhong Hao, David M Wax, Clifton N Murphy, August Rieke, Melissa Samuel, Michael L Linville, Scott W Korte, Rhobert W Evans, Thomas E Starzl, Randall S Prather*, Yifan Dai*, Generation of cloned transgenic pigs rich in omega-3 fatty acids, Nature Biotechnology, 2006, 24(4):435-436.
19. Donna Kolber-Simonds#, Liangxue Lai#, Steven R Watt#, Maria Denaro#, Scott Arn, Monica L Augenstein, Jeffery Betthauser, David B Carter, Julia L Greenstein, Yanhong Hao, Gi-Sun Im, Zhonghua Liu, Greg D Mell, Clifton N Murphy, Kwang-Wook Park, August Rieke, David J J Ryan, David H Sachs, Erik J Forsberg, Randall S Prather, Robert J Hawley*, Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations, Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(19):7335-7340.
20. Liangxue Lai#, Donna Kolber-Simonds, Kwang-Wook Park, Hee-Tae Cheong, Julia L Greenstein, Gi-Sun Im, Melissa Samuel, Aaron Bonk, August Rieke, Billy N Day, Clifton N Murphy, David B Carter, Robert J Hawley, Randall S Prather*, Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning, Science, 2002, 295(5557):1089-1092.