首页 >> 中文 >> 专家人才

专家人才

  • 姓名: 彭广敦
  • 性别: 男
  • 职称: 研究员
  • 学历: 
  • 电话: 
  • 传真: 
  • 电子邮件: peng_guangdun@gibh.ac.cn
  • 通讯地址 广州市科学城开源大道190号

    简历:

  • 2009年毕业于中国科学院上海生物化学与细胞生物学研究所,获得博士学位;美国加州大学洛杉矶分校(UCLA)博士后;回国后在中国科学院上海生物化学与细胞生物学先后担任助理研究员、副研究员。20189月入职中国科学院广州生物医药与健康研究院,担任研究员、课题组长、博士生导师。
     

    研究领域:

  •     17世纪,起始于显微镜的发明以及对微生物的观察,“细胞学说”被提出——“所有生命体皆由细胞组成”,“细胞是生命的基本单元”以及“所有细胞都由此前的细胞变化而来”等核心概念,成为现代生物学的重要基石之一。然而,细胞的类型如何定义、细胞的过去现在和未来如何精确解码—这些细胞的“谱系信息”在漫长的科学研究长河中,一直是科学中的重要和基本问题。捕获细胞谱系的多维信息,尤其是探究其在时间、空间维度的精确动态变化,成了我们探索生命奥秘、解析发育程式、治愈疾病、延缓衰老的基础;对细胞谱系的精准操控,又成了基于细胞治疗、器官重建的未来再生医学应用之不可或缺的前提。本实验室围绕上述问题,主要有以下研究方向:

    研究方向一:高通量自动化单细胞测序技术解析体内干细胞的功能分群与异质性,重绘干细胞的发育起源与命运特化机制

    结合单细胞多组学测序、单细胞谱系示踪以及类器官等手段重点关注胚胎与组织多能干细胞的动态发育分化过程与细胞谱系决定的调控机制。 

    研究方向二:原位空间转录组技术解析空间功能特异的干细胞转录组特征,构建哺乳动物多能干细胞高分辨率的时空动态分子谱系

    发展和利用高通量、高分辨率的空间多组学技术研究组织功能实现的细胞基础,揭示原位、动态、多维度的细胞命运决定与作用网络。

    研究方向三:新型多能干细胞用于体外器官重建与功能修复

    基于细胞谱系的新发现,实现谱系再造、损伤修复与器官的体外重建。


    主要学术成果

        关注胚胎干细胞分化和发育时空动态谱系、生物信息学研究,开发了高通量的自动化单细胞测序方案,大大提高了单细胞转录组测序的便捷、通量、均一性,并具有优异的检测灵敏度、测序质量与灵活性,用于解析不同组织中细胞的功能分群与异质性,利用此技术鉴定了肺上皮具有双潜能的干细胞群体;结合单细胞测序与激光显微切割,建立了原位空间转录组技术(Geo-seq),实现了在组织切片上精确获取原位单个或者少量细胞,解析空间功能特异的细胞转录组特征;首次系统地绘制了小鼠着床后胚胎三个胚层建立过程高分辨率的时空动态分子图谱,揭示了多能干细胞的发育调控网络,并提供了体内发育的空间位置参考坐标系;发现了新型的组织干细胞用于体外器官重建与组织修复,相关工作以第一或通讯作者发表在NatureNature GeneticsDevelopmental CellNature Protocols上,在ScienceCell Stem CellNature Cell Biology等发表共同作者论文或研究综述四十余篇,研究成果获选2019年度中国生命科学十大进展2019年度中国生物信息学十大进展2019年度中国生物信息学十大应用”。


    承担科研项目情况:

  • 中国科学院器官重建与制造”A类战略性先导科技专项

    国家重点研发计划干细胞及转化研究重点专项

    国家重点研发计划“发育编程及其代谢调控”重点专项

    国家高层次人才青年项目

    国家自然科学基金面上项目

    广东省自然科学基金杰出青年项目


    社会任职:

  • 国际干细胞学会(ISSCR)会员


    获奖及荣誉:

  • Sanofi-SIBS 2017 Distinguished Young Faculty

    ISSCR 2017 Poster Award and Travel Award

    国家级人才项目


    代表论著:

  • 近五年第一作者/通讯作者研究论文

    1.     Peng G*#, Suo S#, Cui G#, Yu F#, Wang R, Chen J, Chen S, Liu Z, Chen G, Qian Y, Tam PPL, Han JJ*, Jing N* (2019) Molecular architecture of lineage allocation and tissue organization in early mouse embryo. Nature, 572: 528–532 (F1000 Recommend).

    2.     Liu Q, Liu K, Cui G, Huang X, Yao S, Guo W, Qin Z, Li Y, Yang R, Pu W, Zhang L, He L, Zhao H, Yu W, Tang M, Tian X, Cai D, Nie Y, Hu S, Ren T, Qiao Z, Huang H, Zeng YA, Jing N, Peng G*, Ji H*, Zhou B* (2019) Lung regeneration by multipotent stem cells residing at the bronchioalveolar-duct junction. Nature Genetics, 51: 728–738.

    3.     Peng G#, Suo S#, Chen J#, Chen W, Liu C, Yu F, Wang R, Chen S, Sun N, Cui G, Song L, Tam PPL, Han J-DJ & Jing N (2016) Spatial Transcriptome for the Molecular Annotation of Lineage Fates and Cell Identity in Mid-gastrula Mouse Embryo. Developmental Cell 36, 681–697. (Feature Article, Most read paper)

    4.     Chen J, Suo S, Tam PP, Han J-DJ*, Peng G* & Jing N* (2017) Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq. Nature Protocols, 12, 566–580.

    5.     Peng, G*., Cui, G., Ke, J. and Jing, N*. (2020) Using Single-Cell and Spatial Transcriptomes to Understand Stem Cell Lineage Specification During Early Embryo Development. Annu Rev Genomics Hum Genet, 21, 163-181.

    6.     Xia, Q.#, Cui, G.#, Fan, Y., Wang, X., Hu, G., Wang, L., Luo, X., Yang, L., Cai, Q., Xu, K., Guo, W., Gao, M., Li, Y., Wu, J., Li, W., Chen, J., Qi, H., Peng, G*. and Yao, H*. (2021) RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes. Cell Prolif, 54, e13000.

    7.     Cui G, Jing N, Peng G* (2019) Comments on ‘Molecular architecture of lineage allocation and tissue organization in early mouse embryo’. Journal of Molecular Cell Biology, 11, 1024-1025.

    8.     Peng G, Han JJ (2018) Regulatory network characterization in development: challenges and opportunities. F1000Res, 7.

    9.     Han, X#, Luo, S#, Peng G#, Lu, JY, Cui G, Liu L, Yan P, Yin Y, Liu W, Wang R, Chang Z, Na J, Jing N*, and Shen X* (2018). Mouse knockout models reveal largely dispensable but context-dependent functions of lncRNAs during development. Journal of Molecular Cell Biology. 10:175-178.

    10.  Cui G, Suo S, Wang R, Qian Y, Han JJ, Peng G*, Tam PPL*, Jing N* (2018) Mouse gastrulation: Attributes of transcription factor regulatory network for epiblast patterning. Dev Growth Differ, 60: 463–472

    11.  Peng G (2017) Looking Back: Single-Cell Analysis, Empowering Stem Cell Studies (2017) Cell Stem Cell, 20: 758 (Commentary)

    12.  Peng G, Tam P & Jing N (2017) Lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologies. National Science Review. doi:10.1093/nsr/nwx093

    13.  Peng G* & Jing N* (2017) The genome-wide molecular regulation of mouse gastrulation embryo. Science China Life Science. doi:10.1007/s11427-016-0285-3

    14.  Peng G &Jing N (2016) Early embryo development and lineage determination. Chinese Bulletin of Life Sciences.

     

    已发表共同作者论文

    1.       Niu Y, Sun N, Li C, Lei Y, Huang Z, Wu J, Si C, Dai X, Liu C, Wei J, Liu L, Feng S, Kang Y, Si W, Wang H, Zhang E, Zhao L, Li Z, Luo X, Cui G, Peng G, Izpisúa Belmonte JC, Ji W, Tan T (2019) Dissecting primate early post-implantation development using long-term in vitro embryo culture. Science, 366: doi:10.1126/science.aaw5754

    2.       Shim, W.J., Sinniah, E., Xu, J., Vitrinel, B., Alexanian, M., Andreoletti, G., Shen, S., Sun, Y., Balderson, B., Boix, C., Peng, G., Jing, N., Wang, Y., Kellis, M., Tam, P.P.L., Smith, A., Piper, M., Christiaen, L., Nguyen, Q., Bodén, M. and Palpant, N.J. (2020) Conserved Epigenetic Regulatory Logic Infers Genes Governing Cell Identity. Cell Syst, 11, 625-639.e13.

    3.       Kim, H.J., Osteil, P., Humphrey, S.J., Cinghu, S., Oldfield, A.J., Patrick, E., Wilkie, E.E., Peng, G., Suo, S., Jothi, R., Tam, P.P.L. and Yang, P. (2020) Transcriptional network dynamics during the progression of pluripotency revealed by integrative statistical learning. Nucleic Acids Res, 48, 1828-1842.

    4.       Zhang, K., Yu, F., Zhu, J., Han, S., Chen, J., Wu, X., Chen, Y., Shen, T., Liao, J., Guo, W., Yang, X., Wang, R., Qian, Y., Yang, J., Cheng, L., Zhao, Y., Hui, C.C., Li, J., Peng, G., He, S., Jing, N. and Tang, K. (2020) Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation. Cell Rep, 31, 107521.

    5.       He, D.D., Tang, X.T., Dong, W., Cui, G., Peng, G., Yin, X., Chen, Y., Jing, N. and Zhou, B.O. (2020) C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors. Stem Cell Reports, doi: 10.1016/j.stemcr.2020.03.001

    6.       Yang X, Hu B, Liao J, Qiao Y, Chen Y, Qian Y, Feng S, Yu F, Dong J, Hou Y, Xu H, Wang R, Peng G, Li J, Tang F, Jing N (2019) Distinct enhancer signatures in the mouse gastrula delineate progressive cell fate continuum during embryo development. Cell Research, 29: 911–926

    7.       Zhou Y, Wang X, Song M, He Z, Cui G, Peng G, Dieterich C, Antebi A, Jing N, Shen Y (2019) A secreted microRNA disrupts autophagy in distinct tissues of Caenorhabditis elegans upon ageing. Nature Communications, 10: 4827

    8.       Yu W, Ma X, Xu J, Heumüller AW, Fei Z, Feng X, Wang X, Liu K, Li J, Cui G, Peng G, Ji H, Li J, Jing N, Song H, Lin Z, Zhao Y, Wang Z, Zhou B, Zhang L (2019) VGLL4 plays a critical role in heart valve development and homeostasis. PLoS Genetics, 15: e1007977

    9.       Song W, Wang R, Jiang W, Yin Q, Peng G, Yang R, Yu QC, Chen J, Li J, Cheung TH, Jing N, Zeng YA (2019) Hormones induce the formation of luminal-derived basal cells in the mammary gland. Cell Research, 29: 206–220

    10.    Cheng S, Pei Y, He L, Peng G, Reinius B, Tam PPL, Jing N, Deng Q (2019) Single-Cell RNA-Seq Reveals Cellular Heterogeneity of Pluripotency Transition and X Chromosome Dynamics during Early Mouse Development. Cell Reports, 26: 2593–2607.e3

    11.    Osteil P, Studdert JB, Goh HN, Wilkie EE, Fan X, Khoo PL, Peng G, Salehin N, Knowles H, Han JJ, Jing N, Fossat N, Tam PPL (2019) Dynamics of Wnt activity on the acquisition of ectoderm potency in epiblast stem cells. Development, 146, doi:10.1242/dev.172858

    12.    Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F (2019) A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis. Cell Reports, 27: 1567–1578.e5

    13.    Friedman CE, Nguyen Q, Lukowski SW, Helfer A, Chiu HS, Miklas J, Levy S, Suo S, Han JJ, Osteil P, Peng G, Jing N, Baillie GJ, Senabouth A, Christ AN, Bruxner TJ, Murry CE, Wong ES, Ding J, Wang Y, Hudson J, Ruohola-Baker H, Bar-Joseph Z, Tam PPL, Powell JE, Palpant NJ (2018) Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation. Cell Stem Cell, 23: 586–598.e8

    14.    Christodoulou N, Kyprianou C, Weberling A, Wang R, Cui G, Peng G, Jing N, Zernicka-Goetz M (2018) Sequential formation and resolution of multiple rosettes drive embryo remodelling after implantation. Nature Cell Biology, 20: 1278–1289

    15.    Sibbritt, T., Ip, C. K., Khoo, P. L., Wilkie, E., Jones, V., Sun, J. Q. J., Shen, J. X., Peng, G., Han, J. J., Jing, N., Osteil, P., Ramialison, M., Tam, P. P. L., and Fossat, N. (2018). A gene regulatory network anchored by LIM homeobox 1 (LHX1) for embryonic head development. Genesis, 56: e23246

    16.    Liu C, Wang, R, He Z, Osteil P, Yang X, Chen J, Peng G, Tam P, Jing N (2018), Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports, 11, 43-57.

    17.    Yang X, Hu B, Hou Y, Qiao Y,, Wang R, Chen Y, Qian Y, Feng S, Chen J, Liu C, Peng G, Tang F and Jing N (2018). Silencing of developmental genes by H3K27me3 and DNA methylation reflects the discrepant plasticity of embryonic and extraembryonic lineages. Cell Research 28(5):593-596.

    18.    Liu, C., Peng, G., and Jing, N. (2018). TGF-β signaling pathway in early mouse development and embryonic stem cells. Acta Biochim Biophys Sin. 50, 68-73.

    19.    Zamir L, Singh R, Nathan E, Patrick R, Yifa O, Yahalom-Ronen Y, Arraf AA, Schultheiss TM, Suo S, Han J-DJ, Peng G, Jing N, Wang Y, Palpant N, Tam PP, Harvey RP & Tzahor E (2017) Nkx2.5 marks angioblasts that contribute to hemogenic endothelium of the endocardium and dorsal aorta. elife, 6, e20994.

    20.    Song L, Chen J, Peng G, Tang K & Jing N (2016) Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling. Journal of Biological Chemistry, 291, 15212–15225.

    21.    Tang K, Peng G, Qiao Y, Song L & Jing N (2015) Intrinsic regulations in neural fate commitment. Development, Growth&Differentiation. 57, 109–120.

    22.    Li L, Song L, Liu C, Chen J, Peng G, Wang R, Liu P, Tang K, Rossant J & Jing N (2015) Ectodermal progenitors derived from epiblast stem cells by inhibition of Nodal signaling. Journal of Molecular Cell Biology, 7, 455–465.

    23.    Liu P, Dou X, Peng G, Han J-DJ & Jing N (2015) Genome-wide analysis of histone acetylation dynamics during mouse embryonic stem cell neural differentiation. Genomic Data, 5, 15–16.

    24.    Song L, Sun N, Peng G, Chen J, Han J-DJ & Jing N (2015) Genome-wide ChIP-seq and RNA-seq analyses of Pou3f1 during mouse pluripotent stem cell neural fate commitment. Genomic Data, 5, 375–377.

    25.    Liu P, Dou X, Liu C, Wang L, Xing C, Peng G, Chen J, Yu F, Qiao Y, Song L, Wu Y, Yue C, Li J, Han J-DJ, Tang K & Jing N (2015) Histone deacetylation promotes mouse neural induction by restricting Nodal-dependent mesendoderm fate. Nature Communications, 6, 6830.

    26.    Zhu Q, Song L, Peng G, Sun N, Chen J, Zhang T, Sheng N, Tang W, Qian C, Qiao Y, Tang K, Han J-DJ, Li J & Jing N (2014) The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways. elife, 3, 2.

    27.    Li G, Bahn JH, Lee J-H, Peng G, Chen Z, Nelson SF & Xiao X (2012) Identification of allele-specific alternative mRNA processing via transcriptome sequencing. Nucleic Acids Research 40, e104–e104.

    28.    Bahn JH, Lee J-H, Li G, Greer C, Peng G & Xiao X (2012) Accurate identification of A-to-I RNA editing in human by transcriptome sequencing. Genome Research, 22, 142–150.

    29.    Lee J-H, Gao C, Peng G, Greer C, Ren S, Wang Y & Xiao X (2011) Analysis of transcriptome complexity through RNA sequencing in normal and failing murine hearts. Circulation Research, 109, 1332–1341.

    30.    Kong L, Choi RCY, Xie HQ, Wang X, Peng G, Xie Z, Tsim KWK & Jing N (2011) Expression of the IgSF protein Kirre in the rat central nervous system. Life Sciences, 88, 590–597.

     

    (*: corresponding author)